Coding

Part:BBa_K4613012:Design

Designed by: Yuchen Zhou   Group: iGEM23_NAU-CHINA   (2023-10-08)


C3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1600
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part codes for hydrophilic elastin-like polypeptides(ELPs) fused with triple SpyCatcher sequences, through polymerization by covalent bonding between SpyTag and SpyCatcher, forming polymeric scaffolds. If you want to learn about the detailed introduction of ELPs, you can click the link below. https://parts.igem.org/Part:BBa_K4613010


Source

NAU-CHINA 2023

References

  1. Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nat Commun,2021, 12 (1): 3422.
  2. Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
  3. Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.